Computational Biology Lecture 13: Physical mapping by hybridization

As mentioned before, we have two approaches for physical mapping: Restriction mapping and mapping by hybridization. We covered restriction mapping previously through the two problems of double digest and partial digest. We now look at mapping by hybridization. While restriction mapping involves the mapping of restriction sites (precise short sequences) of a cutting enzyme based on the lengths of the restriction fragments, mapping by hybridization involves the mapping of clones (fragments) based on hybridization data with probes. The mapping of clones in this case is not exact; we try to find a likely overlap of the clones.